Composite

Part:BBa_K2680260:Design

Designed by: Ethan M Jones   Group: iGEM18_William_and_Mary   (2018-10-06)


3G eBFP2


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 47
    Illegal BsaI.rc site found at 783


Design Notes

Sequence from Murray Lab at California Institute of Technology. Used PCR to add William and Mary Pad overhangs, which were used to clone the part into William and Mary Pad backbone. Forbidden restriction sites were checked for and removed if necessary.

Source

"EBFP2 is a basic (constitutively fluorescent) blue fluorescent protein published in 2007 [in Ai et al. 2007], derived from Aequorea victoria. It is reported to be a rapidly-maturing monomer with moderate acid sensitivity." 1

References

1 EBFP2. (n.d.). Retrieved October 16, 2018, from https://www.fpbase.org/protein/ebfp2/

Ai et al. 2007: Ai, H., Shaner, N. C., Cheng, Z., Tsien, R. Y., & Campbell, R. E. (2007). Exploration of New Chromophore Structures Leads to the Identification of Improved Blue Fluorescent Proteins. Biochemistry, 46, 5904-5910. doi:10.1021/bi700199g